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Table 1 Primary and secondary outcome variables

From: Influence of upper-body continuous, resistance or high-intensity interval training (CRIT) on postprandial responses in persons with spinal cord injury: study protocol for a randomised controlled trial

Outcome measure

Analytical method

Metabolites

 Glucose

Plasma glucose concentration will be assessed using an automated analyser (Randox RX Daytona, Co. Antrim, UK).

 Lipids

Serum markers of total triglycerides, total cholesterol, NEFA, HDL-C and LDL-C will also be assessed using an automated analyser and commercially available immunoassays (Randox Laboratories, Co. Antrim, UK).

Hormones

 Insulin

Determined using ELISA (Mercodia AB, Uppsala, Sweden). Variables:

(i) incremental area under the curve (iAUC) [54];

(ii) insulin sensitivity index (ISIMatsuda) [55];

(iii) Homeostasis Model Assessment (HOMA-2) model [56]

Inflammatory markers

 Il-6

Baseline and post-exercise measures of IL-6 will be assessed using ELISA (Quantikine HS, R&D Systems Inc., Abingdon, UK)

Energy expenditure

 Total energy expenditure  (kcal/min)

Total energy expenditure and substrate oxidation rates will be determined from expired gas analysis, at rest and during exercise, using indirect calorimetric methods, as previously described [47]

 CHO-ox (g/min)

 Fat-ox (g/min)

  1. Abbreviations: CHO carbohydrate, ELISA enzyme-linked immunosorbent assay, HDL-C high-density lipoprotein cholesterol, IL interleukin, LDL-C low-density lipoprotein cholesterol, NEFA non-esterified fatty acids