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Table 1 Post-hoc characterization of leukocyte differentiation markers

From: Multicenter double blind trial of autologous bone marrow mononuclear cell transplantation through intracoronary injection post acute myocardium infarction – MiHeart/AMI study

Phenotype

Intended designation

1. CD45loCD34+

ISHAGE protocol

2. Lin-CD45loCD34+

Hematopoietic progenitors

3. Lin-CD45loCD34+CD133+CD117+

Hematopoietic stem cells*

4. Lin-CD45-CD133+CD31+CD144+KDR+

Endothelial precursor*

5. Lin-CD45-CD133-CD31+CD144+KDR+

Endothelial cell*

6. CD45+CD10+CD33+HLA-DR-CD45RA-

Myeloid precursor*

7. CD45+CD10+CD33-HLA-DR+CD45RA+

Lymphoid precursor*

8. CD45-CD133+CD146+CD144+CD166+

Mesenchymal progenitor*

9. CD45-CD133-CD146+CD73+CD166+

Mesenchymal cell*

10. CD45+CD10+CD34+CD19-CD38-TdT+IgM-

pre-pro-B intermediary

   10.1. CD45+CD10+CD34+CD19+CD38-TdT+IgM-

pro-B/pre-B I intermediary

   10.2. CD45+CD10+CD34-CD19+CD38-TdT-IgM-

Pre-B II intermediary

   10.3. CD45+CD10-CD34-CD19+CD38+TdT-IgM+CD24hi

Immature B LΦ

   10.4. CD45+CD10-CD34-CD19+CD38+TdT-IgM+

Naïve B LΦ

   10.5. CD45+CD10-CD34-CD19+CD38+TdT-IgM+CD24loCD69+

Mature B LΦ

11. CD45+CD34+CD38-CD1a-CD3-CD4-CD8-

LΦ-T DN1

   11.1. CD45+CD34+CD38+CD1a-CD3-CD4-CD8-

LΦ-T DN2

   11.2. CD45+CD34+CD38+CD1a+CD3-CD4-CD8-

LΦ-T DN3

   11.3. CD45+CD34+CD38+CD1a+CD3loCD4+CD8-

LΦ-T DN4-ISP

   11.4. CD45+CD34+CD38+CD3-CD4+CD8+

LΦ-T DP

   11.5. CD45+CD34+CD38+CD3+CD4+CD8+

LΦ-T DP-CD3

12. CD45+CD34+CD3+CD4+CD8-

LΦ-T CD4

13. CD45+CD34+CD3+CD4-CD8+

LΦ-T CD8

14. CD45+CD122+CD56+CD3-

NK cells

15. CD45+CD66acde+

Neutrophils/Basophils

16. CD45+CD41a+

Megakaryocytes

17. CD45+CD66acde-CD41a-CD14+CD16+

Monocytes/Macrophages

  1. *The intended designation to some phenotypes will be more deeply explored with additional markers like CD117, CD31, CD105, CD106, CD166 and others of interest. Besides, cell subpopulations also will be sorted to perform supplementary function assays.